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The Low Molecular Weight Marker is a set of 14 bands between 10 - 100 nucleotides, designed for size determination of single-stranded oligonucleotide fragments.
The Low Molecular Weight Marker is suitable for use in either polyacrylamide or agarose gel electrophoresis. The recommended concentration for agarose is 2.5% and for polyacrylamide is 12 – 15%. This marker should be diluted with TE buffer and the supplied Gel Loading Buffer to the desired loading concentration.
The Low Molecular Weight Marker can be used to generate a 5’-end radiolabeled marker for size comparison of small, single-stranded oligonucleotide fragments such as miRNA, siRNA, tRNA, snRNA, snoRNA, or ssDNA. The Low Molecular Weight Marker is also useful when the molecule of interest is less than 100 nucleotides in length. This marker is not designed for precise quantification of nucleic acid mass and should only be used as a size marker.
Storage: Ship on ice. Store at 4°C or at -20°C for long term storage.