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4-Amino-3-hydrazino-5-mercapto-1,2,4-triazole is a reagent used in the determination of aldehydes and other reactive chemicals.was used in purpald assay for determination of bacterial polysaccharides containing substituted or unsubstituted glycol in residues such as glycerol, ribitol, arabinitol, furanosyl galactose and sialic acid. It was used as chromogen during the determination of catalase activity in small tissue samples. It was used as reagent in determination of formaldehyde. It was used for derivatization of neutral monosaccharides during HPLC.
Kunihiro KamatA; Misako Takahashi; Kiyoshi Terajima and Motohiro Nishijima. Spectrophotometric determination of sodium chondroitin sulfate in eye drops after derivatization with 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole.Analyst.1995, 120 2755-2758.
M. J. Del Nozala; J. L. Bernala; V. Hernandeza; L. Toribioa & R. Mendezb. Purpald (4-Amino-3-hydrazino-5-mercapto-1, 2, 4-triazole) as a Reagent for Post-Column Derivatization of Neutral Monosaccharides in High Pressure Liquid Chromatography.Journal of Liquid Chromatography.1993, 16 1105-1116.
Reagent for the detection and classification of aldehydes: Chem. Commun., 1719 (1970); J. Chem. Educ., 55, 206 (1978). The reagent gives no color reaction with ketones.
Hazard Statements: H228-H315-H319-H335
Flammable solid. Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.
Precautionary Statements: P210-P261-P280a-P305+P351+P338-P405-P501a
Keep away from heat/sparks/open flames/hot surfaces. - No smoking. Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves and eye/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.