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3326-32-7 - Fluorescein isothiocyanate, isomer 1, 95% - L09319 - Alfa Aesar

L09319 Fluorescein isothiocyanate, isomer 1, 95%

CAS Number
3326-32-7
Synonyms

Size Price ($) Quantity Availability
50mg 28.43
250mg 82.71
1g 224.54
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Fluorescein isothiocyanate, isomer 1, 95%

MDL
MFCD00005063
EINECS
222-042-0

Chemical Properties

Formula
C21H11NO5S
Formula Weight
389.39
Melting point
>360°
Sensitivity
Moisture Sensitive
Solubility
Slightly soluble in DMSO, ethanol, methanol DMF and acetone

Applications

Fluorescein isothiocyanate is used as fluorescent labeling reagent for proteins and for rapid identification of pathogens in fluorescent antibody technique. It is also used as a reagent for microsequencing of proteins and peptides (HPLC). On celite it is used for the labeling of fibrinogen.

Notes

Incompatible with strong oxidizing agents, strong bases and amines.

Literature References

Reagent for fluorescent labelling of proteins: Anal. Biochem., 20, 178 (1968).

Guilini, C.; Baehr, C.; Schaeffer, E.; Gizzi, P.; Rufi, F.; Haiech, J.; Weiss, E.; Bonnet, D.; Galzi, J. New Fluorescein Precursors for Live Bacteria Detection. Anal. Chem. 2015, 87 (17), 8858-8866.

Chen, Y.; Yang, C.; Li, T.; Zhang, M.; Liu, Y.; Gauthier, M. A.; Zhao, Y.; Wu, C. The Interplay of Disulfide Bonds, α-Helicity, and Hydrophobic Interactions Leads to Ultrahigh Proteolytic Stability of Peptides. Biomacromolecules 2015, 16 (8), 2347-2355.

GHS Hazard and Precautionary Statements

Hazard Statements: H334-H317

May cause allergy or asthma symptoms or breathing difficulties if inhaled. May cause an allergic skin reaction.

Precautionary Statements: P260-P262-P280g-P309-P310

Do not breathe dust/fume/gas/mist/vapours/spray. Do not get in eyes, on skin, or on clothing. Wear protective gloves. IF exposed or if you feel unwell: Immediately call a POISON CENTER or doctor/physician.

Other References

Beilstein
1407295
Harmonized Tariff Code
2932.20
TSCA
Yes

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