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Fast Blue BB salt is used as a general substrate for β-glycosidase. Two moles of 6-bromo-2-napthol-β-d-glucopyranoside are cleaved by β-glucosidases into 2 moles of d-glucose and 2 moles of 6-bromo-2-napthol. The 2 moles of 6-bromo-2-napthol formed are coupled with 1 mole of Fast Blue BB salt [4-benzoylamino-2,5-diethoxybenzenediazonium chloride hemi (zinc chloride) salt], resulting in 2 moles of the corresponding dye of 6-bromo-2-naphthol (reddish color). It is a commonly used stain to observe myelin under light microscopy.
Helene Breitschopf.; Gerda Suchanek.; Robert M. Gould.; David R. Colman.; Hans Lassmann. In situ hybridization with digoxigenin-labeled probes: sensitive and reliable detection method applied to myelinating rat brain.Acta Neuropathologica. 1992, 84 (6),581-587 .
Hazard Statements: H302-H351
Harmful if swallowed. Suspected of causing cancer.
Precautionary Statements: P201-P280-P301+P312a-P308+P313-P405-P501a
Obtain special instructions before use. Wear protective gloves/protective clothing/eye protection/face protection. IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell. IF exposed or concerned: Get medical advice/attention. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.