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Fast Blue BB salt is used as a general substrate for β-glycosidase. Two moles of 6-bromo-2-napthol-β-d-glucopyranoside are cleaved by β-glucosidases into 2 moles of d-glucose and 2 moles of 6-bromo-2-napthol. The 2 moles of 6-bromo-2-napthol formed are coupled with 1 mole of Fast Blue BB salt [4-benzoylamino-2,5-diethoxybenzenediazonium chloride hemi (zinc chloride) salt], resulting in 2 moles of the corresponding dye of 6-bromo-2-naphthol (reddish color). It is a commonly used stain to observe myelin under light microscopy.
Helene Breitschopf.; Gerda Suchanek.; Robert M. Gould.; David R. Colman.; Hans Lassmann. In situ hybridization with digoxigenin-labeled probes: sensitive and reliable detection method applied to myelinating rat brain.Acta Neuropathologica. 1992, 84 (6),581-587 .
Hazard Statements: H315-H319-H335
Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.
Precautionary Statements: P261-P264b-P271-P280-P302+P352-P304+P340-P305+P351+P338-P312-P332+P313-P337+P313-P362-P501c
Avoid breathing dust/fume/gas/mist/vapours/spray. Wash face, hands and any exposed skin thoroughly after handling Use only outdoors or in a well-ventilated area. Wear protective gloves/protective clothing/eye protection/face protection. IF ON SKIN: Wash with plenty of soap and water. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Call a POISON CENTER or doctor/physician if you feel unwell. If skin irritation occurs: Get medical advice/attention. If eye irritation persists: Take off contaminated clothing and wash before reuse. Dispose of contents/ container to an approved waste disposal plant