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The activities of free and immobilized PPL were determined by spectrophotometric assay using 4-nitrophenyl palmitate (pNPP) as substrate.
E. Ranjbakhsh,; A.K. Bordbar,; M. Abbasia,; A.R. Khosropoura,; E. Shams. Supuran. Enhancement of stability and catalytic activity of immobilized lipase on silica-coated modified magnetite nanoparticles. Chemical Engineering Journal. 2012, 179 272-276.
C K Tu, H G Thomas,; G C Wynns,; D N Silverman. Hydrolysis of 4-nitrophenyl acetate catalyzed by carbonic anhydrase III from bovine skeletal muscle. The Journal of Biological Chemistry, . 1986 , 261 10100-10103..