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Pyranine is used as a fluorescent pH indicator with emission change in the physiological pH range. It acts as a dye in optical sensor applications. It finds application as a coloring agent and biological stain. It is also used in the measurement of pH of intracellular fluid in the cells.
Nairn, J. J.; Forster, W. A.; Zydenbos, S. M. Photostability of pyranine and suitability as a spray drift tracer. N. Z. Plant Prot. 2015, 68, 32-37.
Schäferling, M.; Lang, T.; Schnettelker, A. Turn-on fluorescence probes based on pyranine/viologen charge-transfer complexes for the determination of nucleotides. J. Lumin. 2014, 154, 458-464.
Hazard Statements: H315-H319-H335
Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.
Precautionary Statements: P261-P264b-P271-P280-P302+P352-P304+P340-P305+P351+P338-P312-P332+P313-P337+P313-P362-P501c
Avoid breathing dust/fume/gas/mist/vapours/spray. Wash face, hands and any exposed skin thoroughly after handling Use only outdoors or in a well-ventilated area. Wear protective gloves/protective clothing/eye protection/face protection. IF ON SKIN: Wash with plenty of soap and water. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Call a POISON CENTER or doctor/physician if you feel unwell. If skin irritation occurs: Get medical advice/attention. If eye irritation persists: Take off contaminated clothing and wash before reuse. Dispose of contents/ container to an approved waste disposal plant