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Fluorescein is used as a fluorescent tracer for many applications including in a type of dye laser as the gain medium, in forensics and serology to detect latent blood stains and in dye tracing. It is used to localise multiple muscular ventricular septal defects during open heart surgery and confirm the presence of any residual defects. It is applied to teeth to reveal plaque.
Akram, A. C.; Noman, S.; Moniri-Javid, R.; Gizicki, J. P.; Reed, E. A.; Singh, S. B.; Basu, A. S.; Banno, F.; Fujimoto, M.; Ram, J. L. Development of an automated ballast water treatment verification system utilizing fluorescein diacetate hydrolysis as a measure of treatment efficacy. Water Res. 2015, 70, 404-413.
Tichauer, K. M.; Guthrie, M.; Hones, L.; Sinha, L.; St. Lawrence, K.; Kang-Mieler, J. J. Quantitative retinal blood flow mapping from fluorescein videoangiography using tracer kinetic modeling. Opt. Lett. 2015, 40 (10), 2169-2172.
Hazard Statements: H319
Causes serious eye irritation.
Precautionary Statements: P260-P201-P280i-P304+P340-P405-P501a
Do not breathe dust/fume/gas/mist/vapours/spray. Obtain special instructions before use. Wear eye/face protection. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.