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2-Succinimido-1,1,3,3-tetramethyluronium tetrafluoroborate (TSTU) acts as an efficient coupling agent through rapid activation of carboxylic acids to form N-succinimidyl active esters, which react with primary amines to form carboxamides. It is effective even in the presence of water. TSTU is selective towards amines in the presence of alcoholic group. It is employed as a coupling regent in the synthesis of peptides. With amine-protected alpha-amino acids, it brings about coupling without significant epimerization. It is useful for linking of sensor molecules with amino groups to amino-functionalized AFM (atomic force microscope) tips.
Yang, Q.; Wang, H.; Lan, X.; Cheng, B.; Chen, S.; Shi, H.; Xiao, H.; Ma, Y. Reflection-mode micro-spherical fiber-optic probes for in vitro real-time and single-cell level pH sensing. Sens. Actuators B 2015, 207, 571-580.
Yang, Q.; Wang, H.; Chen, S.; Lan, X.; Xiao, H.; Shi, H.; Ma, Y. Fiber-Optic-Based Micro-Probe Using Hexagonal 1-in-6 Fiber Configuration for Intracellular Single-Cell pH Measurement. Anal. Chem. 2015, 87 (14), 7171-7179.
Hazard Statements: H302-H315-H319-H335
Harmful if swallowed. Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.
Precautionary Statements: P261-P264b-P270-P271-P280-P301+P312-P302+P352-P304+P340-P305+P351+P338-P312-P330-P332+P313-P337+P313-P362-P501c
Avoid breathing dust/fume/gas/mist/vapours/spray. Wash face, hands and any exposed skin thoroughly after handling Do not eat, drink or smoke when using this product. Use only outdoors or in a well-ventilated area. Wear protective gloves/protective clothing/eye protection/face protection. IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell. IF ON SKIN: Wash with plenty of soap and water. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Rinse mouth. If skin irritation occurs: Get medical advice/attention. If eye irritation persists: Take off contaminated clothing and wash before reuse. Dispose of contents/ container to an approved waste disposal plant