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Western Blot and ELISA Reagents for Immunology Research - Alfa Aesar

Western Blot and ELISA Reagents

Western blotting and ELISA and are analytical techniques used to detect specific proteins in a biological sample. Both techniques rely on immunodetection of an antibody to a specific protein. Western blotting is used to detect a specific target protein out of a complex protein mixture. A protein mixture is separated by polyacrylamide gel electrophoresis before transfer to a membrane. Antibody binding to the target protein on the membrane can then be detected by various methods. Like Western blots, ELISA is used to detect peptides in a mixture. ELISA also allows quantification of the antigen in question. With ELISA and Western blotting, the presence and/or amount of the antigen is determined directly or indirectly by an enzyme (usually horseradish peroxidase or alkaline phosphatase)-, biotin- or fluorophore-conjugated antibody. Depending on the substrates used, the conjugated enzymes can be detected by colorimetric, fluorescent or chemiluminescent techniques. Alfa Aesar offers a variety of reagents for Western blotting including enzyme substrates and blocking, transfer and washing buffers.

  • 3,3',5,5'-Tetramethylbenzidine, 98%
  • 3,3',5,5'-Tetramethylbenzidine aq. soln.
  • 3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate, 98%
  • 3,3',5,5'-Tetramethylbenzidine soln., Ready-to-Use, high sensitivity
  • 3,3',5,5'-Tetramethylbenzidine soln., Ready-to-Use, precipitating, high sensitivity
  • 3,3',5,5'-Tetramethylbenzidine soln., Ready-to-Use, precipitating, standard sensitivity
  • 3,3',5,5'-Tetramethylbenzidine soln., Ready-to-Use, standard sensitivity
  • 3-Amino-9-ethylcarbazole, 95%
  • 3-Indoxyl phosphate disodium salt
  • BCA Protein Assay Kit
  • BLOTTO
  • BLOTTO and Tween 20 in PBS
  • BLOTTO and Tween 20 in PBS, with 0.02% sodium azide
  • BLOTTO and Tween 20 in TBS
  • BLOTTO and Tween 20 in TBS, with 0.02% sodium azide
  • BLOTTO antifoam in PBS
  • BLOTTO antifoam in PBS, with 0.02% sodium azide
  • BLOTTO antifoam in TBS
  • BLOTTO antifoam in TBS, with 0.02% sodium azide
  • BLOTTO in PBS
  • BLOTTO in PBS, with 0.02% sodium azide
  • BLOTTO in TBS
  • BLOTTO in TBS, with 0.02% sodium azide
  • Bolton-Hunter Reagent
  • BSA blocking buffer, 3% in PBS
  • BSA blocking buffer, 3% in PBS, with 0.02% sodium azide
  • BSA blocking buffer, 3% in PBS, with 0.05% Tween-20
  • BSA blocking buffer, 3% in TBS
  • BSA blocking buffer, 3% in TBS, with 0.05% Tween-20
  • BSA blocking buffer, 5% in PBS
  • BSA blocking buffer, 5% in PBS, with 0.05% Tween-20
  • BSA blocking buffer, 5% in TBS
  • BSA blocking buffer, 5% in TBS, with 0.02% sodium azide
  • BSA blocking buffer, 5% in TBS, with 0.05% Tween-20
  • Casein blocking buffer, 3% in PBS
  • Casein blocking buffer, 3% in PBS, with 0.02% sodium azide
  • Casein blocking buffer, 3% in TBS
  • Casein blocking buffer, 3% in TBS, with 0.02% sodium azide
  • C-Reactive Protein
  • Cyclic AMP Enzyme Immunoassay Kit, BT-730
  • Cyclic GMP Enzyme Immunoassay Kit, BT-740
  • Denhardt's solution (100X)
  • Denhardt's solution (50X)
  • Diatrizoic acid
  • FCS blocking buffer in PBS
  • FCS blocking buffer in TBS
  • Fibronectin, human, Enzyme Immunoassay Kit, BT-700
  • Gelatin and Tween 20 in PBS
  • Gelatin and Tween 20 in PBS, with 0.02% sodium azide
  • Gelatin and Tween 20 in TBS

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