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91-95-2 - 3,3'-Diaminobenzidine, tablets - J60972 - Alfa Aesar

J60972 3,3'-Diaminobenzidine, tablets

Número CAS
91-95-2
Nombre Alternativo

Tamaño Precio ($) Cantidad Disponibilidad
50each 109,00
100each 207,00
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3,3'-Diaminobenzidine, tablets

MDL
MFCD00007725
EINECS
202-110-6

Propiedades químicas

Fórmula
C12H14N4
Peso molecular
214.27
Formulario
Tablets
Sensibilidad
Light Sensitive
Solubilidad
Soluble in water (partly).

Aplicaciones

3,3'-Diaminobenzidine is used to detect fingerprints in blood. 3,3'-Diaminobenzidine is used in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. Slides stained with DAB may be coverslipped in the traditional manner and stored for future reference. Many enhancement methods have been reported for DAB involving different buffer conditions, the addition of metal ions and post-treatment applications. DAB has been used in immunohistochemical staining of nucleic acids and proteins. Application of the silver-gold intensified 3,3? -diaminobenzidine chromogen to the light and electron microscopic detection of the luteinizing hormone-releasing hormone system of the rat brain. 3, 3?-diaminobenzidine (DAB)-H2O2-horseradish peroxidase (HRP) has been presented and used for the sensitive detection of carcinoembryonic antigen (CEA) in human serum.

Notas

Each tablet contains 5mg of DAB, each tablet weighs 240mg. One tablet will yield 10 ml of substrate solution, enough to effectively detect 50cm² of membrane or about 20 slides. Insoluble product. Reaction Condition: Dissolve one 5mg tablet in 10 ml of 50mM Tris, 0.1M NaCl, pH 7.6 and 0.01% hydrogen peroxide. The solution may be filtered through a 0.2µm filter immediately prior to use if necessary. The presence of nickel or cobalt increases sensitivity.

Referencias documentales

Zs. Liposits,; Gy. Sétaló, B. Flerkó.Application of the silver-gold intensified 3,3? -diaminobenzidine chromogen to the light and electron microscopic detection of the luteinizing hormone-releasing hormone system of the rat brain . Neuroscience. 1984, 13 (2), 513-525.

Volker Herzog,; H.Dariush Fahimi. A new sensitive colorimetric assay for peroxidase using 3,3?-diaminobenzidine as hydrogen donor . Analytical Biochemistry. 1973 , 55 (2), 554-562.

Indicaciones de peligro y de precaución del GHS

Indicaciones de peligro (UE): H341-H350

Suspected of causing genetic defects. May cause cancer.

Indicaciones de precaución: P201-P280-P202-P308+P313-P405-P501a

Obtain special instructions before use. Wear protective gloves/protective clothing/eye protection/face protection. Do not handle until all safety precautions have been read and understood. IF exposed or concerned: Get medical advice/attention. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.

Otras referencias

Beilstein
1212988
Código de tarifa arancelaria unificado
2921.59
TSCA
Yes
RTECS
DV8750000

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