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39445-21-1 - Elastase, porcine pancreas - E.C. 3.4.21.36 - Pancreatopeptidase E - J61874 - Alfa Aesar

J61874 Elastase, porcine pancreas

Número CAS
39445-21-1
Nombre Alternativo
E.C. 3.4.21.36
Pancreatopeptidase E

Tamaño Precio ($) Cantidad Disponibilidad
25mg 86,30
100mg 261,00
1g 1190,00
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Elastase, porcine pancreas

MDL
MFCD00130998
EINECS
254-453-6

Propiedades químicas

Formulario
Lyophilized powder, MW 25000
Solubilidad
Soluble in water or Tris buffer up to 2.5mg/ml. Avoid high salt concentrations (ie >50mM) if salt is necessary in the buffer. Unstable in solution at pH <3.5.

Aplicaciones

This enzyme can be used alone or in combination with other proteases for protein analysis by mass spectrometry and other applications. Elastase, is used in tissue dissociation because elastin is found in highest concentrations in the elastic fibers of connective tissues, elastase is frequently used to dissociate tissues that contain extensive intercellular fiber networks. For this purpose, it is usually used with other enzymes such as collagenase, trypsin, and chymotrypsin, membrane protein solubilization, protein sequence studies.

Notas

Minimum 3 units per mg protein. One unit cleaves one micromole of N-succinyl-L-alanyl-L-alanyl-L-alanine-p-nitroanilide per minute at 25 degrees and pH 8.0

Referencias documentales

RD Hautamaki.; DK Kobayashi.; RM Senior.; SD Shapiro. Requirement for macrophage elastase for cigarette smoke-induced emphysema in mice. Science. 1997, 277, (5334), 2002-2004 .

J Bieth.; B Spiess.; CG Wermuth. The synthesis and analytical use of a highly sensitive and convenient substrate of elastase. Biochemical medicine. 1974, 11, (4), 350-357.

Indicaciones de peligro y de precaución del GHS

Indicaciones de peligro (UE): H315-H319-H334-H335

Causes skin irritation. Causes serious eye irritation. May cause allergy or asthma symptoms or breathing difficulties if inhaled. May cause respiratory irritation.

Indicaciones de precaución: P284-P261-P280a-P305+P351+P338-P405-P501a

Wear respiratory protection. Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves and eye/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.

Otras referencias

Merck
14,3535
Código de tarifa arancelaria unificado
3507.90
TSCA
No
RTECS
TS5052000

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