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145224-94-8 - MES, 0.5M buffer soln., pH 5.0 - J62081 - Alfa Aesar

J62081 MES, 0.5M buffer soln., pH 5.0

Número CAS
145224-94-8
Nombre Alternativo

Tamaño Precio ($) Cantidad Disponibilidad
100ml 48,20
250ml 56,16
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MES, 0.5M buffer soln., pH 5.0

MDL
MFCD00283848

Propiedades químicas

Formulario
Liquid
Solubilidad
Soluble in water (0.5 M at 20°C).

Aplicaciones

MES, is often used as a running buffer for resolving very small proteins on bis-tris gels, also used as a biological buffer in plant cell cultures. It is also been used in various protein characterization assays. This sterile solution suitable for cell culture and molecular biology applications. It is also used as a buffering agent in biology and biochemistry.

Notas

Solutions are stable at 2-8°C for months. Sterilize by filtration through 0.2 mm filters. Autoclaving is not recommended for any sulfonic acid buffer. If buffers must be nuclease-free, treat the water first, then add the buffer after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown.

Referencias documentales

BG Bugbee.; FB Salisbury. An evaluation of MES (2 (N-Morpholino) ethanesulfonic acid) and amberlite IRC-50 as pH buffers for nutrient solution studies. Journal of plant nutrition. 1985, 8, ( 7), 567-583.

MJ Alowitz.; MM Scherer . Kinetics of nitrate, nitrite, and Cr (VI) reduction by iron metal. Environmental Science & Technology. 2002, 36, (3), 299-306.

Indicaciones de peligro y de precaución del GHS

Indicaciones de peligro (UE): H315-H319

Causes skin irritation. Causes serious eye irritation.

Indicaciones de precaución: P280-P264-P305+P351+P338-P362-P321-P332+P313-P337+P313-P302+P352

Wear protective gloves/protective clothing/eye protection/face protection. Wash thoroughly after handling. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Take off contaminated clothing and wash before reuse. Specific treatment (see label). If skin irritation occurs: Get medical advice/attention. If eye irritation persists: IF ON SKIN: Wash with plenty of soap and water.

Otras referencias

Código de tarifa arancelaria unificado
2934.99
TSCA
Yes

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