5alpha-Cholestan-3beta-ol is used as a standard in lipid analysis using HPLC. It acts as a derivitized steroid compound. It is used in agrochemical, pharmaceutical and dyestuff field .
Benjamin L Stottrup; Sarah L Keller. Phase behavior of lipid monolayers containing DPPC and cholesterol analogs.Biophysical Journal.2006, 90 (9), 3176-3183.
S Shefer; S Milch; E H Mosbach. Biosynthesis Of 5-Alpha-Cholestan-3-Beta-Ol In The Rabbit And Guinea Pig.Journal of Biological Chemistry.1964, 239 1731-1736.