Tris(2-carboxyethyl)phosphine hydrochloride is widely used as a reducing agent to reduce disulfide bonds in proteins and trans-4,5-dihydroxy-1,2-dithiane. It is involved in the reduction of sulfoxides, sulfonyl chlorides, N-oxides and azides. It plays an important role in the tissue homogenization process for RNA isolation. It acts as a chelating agent and forms complexes with various heavy metal ions such as Zn(II), Cd(II), Pb(II), and Ni(II). Furthermore, it is useful in the labeling of cysteine residues with maleimides.
La, M.; Feng, Y.; Yang, C.; Chen, C. Performances of p-Aminophenol Redox Cycling by Thiols and Tris (2-carboxyethyl) phosphine on Cysteamine-and 3-Mercaptopropionic acid-Covered Gold Electrodes. Int. J. Electrochem. Sci. 2014, 9, 6985-6992.
Miralles, G.; Verdie?, P.; Puget, K.; Maurras, A.; Martinez, J.; Subra, G. Microwave-mediated reduction of disulfide bridges with supported (Tris (2-carboxyethyl) phosphine) as resin-bound reducing agent. ACS Comb. Sci. 2013, 15 (4), 169-173.
Mentions de danger (UE): H314-H318
Causes severe skin burns and eye damage. Causes serious eye damage.
Mentions de prudence: P260u-P303+P361+P353-P305+P351+P338-P301+P330+P331-P405-P501a
Do not breathe dusts or mists. IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. Rinse skin with water/shower. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF SWALLOWED: Rinse mouth. Do NOT induce vomiting. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.