Methyl 4-ethynylbenzoate is used as pharmaceutical intermediate.
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Ronald A.Forsch; Sherry F.Queener; Andre Rosowsky. Preliminary in vitro studies on two potent, water-soluble trimethoprim analogues with exceptional species selectivity against dihydrofolate reductase from Pneumocystis carinii and Mycobacterium avium. Bioorganic & Medicinal Chemistry Letters. 2004, 14, (7),1811-1815