Laemmli SDS sample buffer is used for protein sample preparation, which is used in the Laemmli SDS-PAGE system. It is a widely used system for protein electrophoresis. Further, it is employed in the polyacrylamide gel analysis of proteins. In addition to this, it is utilized to reduce disulfide bonds in order to denature proteins.
Lazar, E.; Peterfi, Z.; Sirokmany, G.; Kovacs, H. A.; Klement, E.; Medzihradszky, K. F.; Geiszt, M. Structure-function analysis of peroxidasin provides insight into the mechanism of collagen IV crosslinking. Free Radical Biol. Med. 2015, 83, 273-282.
Saul, J.; Petritis, B.; Sau, S.; Rauf, F.; Gaskin, M.; Reynolds, B. O.; Mineyev, I.; Magee, M.; Chaput, J.; Qiu, J.; LaBaer, J. Development of a full-length human protein production pipeline. Protein Sci. 2014, 23 (8), 1123-1135.
Mentions de danger (UE): H227-H303-H312-H315-H318
Combustible liquid. May be harmful if swallowed. Harmful in contact with skin. Causes skin irritation. Causes serious eye damage.
Mentions de prudence: P210u-P280-P305+P351+P338-P310a-P403+P235-P501a
Keep away from heat/sparks/open flames/hot surfaces. - No smoking. Wear protective gloves/protective clothing/eye protection/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER/doctor Store in a well-ventilated place. Keep cool. Dispose of contents/container in accordance with local/regional/national/international regulations.