Endonuclease that splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide and removal of DNA from protein samples. Also used in is used to nick DNA, as a first step to incorporate labeled bases into DNA. The enzyme from Sigma has been used in the nuclease stock along with RNAse during cell lysate preparation of Madin-Darby canine kidney (MDCK) II cell lines.
David B N Lee; Nora Jamgotchian; Suni G Allen; Frederick W K Kan; Irene L Hale. Annexin A2 heterotetramer: role in tight junction assembly. American Journal of Physiology - Renal Physiology. 2004 287(3), F481-F491.
Y Shirako; J H Strauss. Regulation of Sindbis virus RNA replication: uncleaved P123 and nsP4 function in minus-strand RNA synthesis, whereas cleaved products from P123 are required for efficient plus-strand RNA synthesis. Journal of Virology. 1994 68(3), 1874-1885.