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J60972 3,3'-Diaminobenzidine, tablets

Numéro de CAS
91-95-2
Synonymes

Stock No. Conditionnement Prix ($) Quantité Disponibilité
J60972-KW 50each 195,00
J60972-KX 100each 272,00
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3,3'-Diaminobenzidine, tablets

MDL
MFCD00007725
EINECS
202-110-6

Propriétés chimiques

Formule
C12H14N4
Poids moleculaire
214.27
Forme
Tablets
Storage & Sensitivity
Store at -20°C. Light Sensitive.
Solubilité
Soluble in water (partly).

Applications

3,3'-Diaminobenzidine is used to detect fingerprints in blood. 3,3'-Diaminobenzidine is used in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. Slides stained with DAB may be coverslipped in the traditional manner and stored for future reference. Many enhancement methods have been reported for DAB involving different buffer conditions, the addition of metal ions and post-treatment applications. DAB has been used in immunohistochemical staining of nucleic acids and proteins. Application of the silver-gold intensified 3,3? -diaminobenzidine chromogen to the light and electron microscopic detection of the luteinizing hormone-releasing hormone system of the rat brain. 3, 3?-diaminobenzidine (DAB)-H2O2-horseradish peroxidase (HRP) has been presented and used for the sensitive detection of carcinoembryonic antigen (CEA) in human serum.

Notes

Each tablet contains 5mg of DAB, each tablet weighs 240mg. One tablet will yield 10 ml of substrate solution, enough to effectively detect 50cm² of membrane or about 20 slides. Insoluble product. Reaction Condition: Dissolve one 5mg tablet in 10 ml of 50mM Tris, 0.1M NaCl, pH 7.6 and 0.01% hydrogen peroxide. The solution may be filtered through a 0.2µm filter immediately prior to use if necessary. The presence of nickel or cobalt increases sensitivity.

Références bibliographiques

Zs. Liposits,; Gy. Sétaló, B. Flerkó.Application of the silver-gold intensified 3,3? -diaminobenzidine chromogen to the light and electron microscopic detection of the luteinizing hormone-releasing hormone system of the rat brain . Neuroscience. 1984, 13 (2), 513-525.

Volker Herzog,; H.Dariush Fahimi. A new sensitive colorimetric assay for peroxidase using 3,3?-diaminobenzidine as hydrogen donor . Analytical Biochemistry. 1973 , 55 (2), 554-562.

Mentions de danger et de prudence du SGH

Mentions de danger (UE): H341-H350

Suspected of causing genetic defects. May cause cancer.

Mentions de prudence: P201-P280-P202-P308+P313-P405-P501a

Obtain special instructions before use. Wear protective gloves/protective clothing/eye protection/face protection. Do not handle until all safety precautions have been read and understood. IF exposed or concerned: Get medical advice/attention. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.

Autres références

Beilstein
1212988
Code tarifaire harmonisé
2921.59
TSCA
Yes
RTECS
DV8750000

Recommandé

Consulté récemment

Produits chimiques

Sciences de la vie

Métaux et matériaux

Catalyseurs

Analytique et matériel de laboratoire