3,3'-Diaminobenzidine is used to detect fingerprints in blood. 3,3'-Diaminobenzidine is used in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. Slides stained with DAB may be coverslipped in the traditional manner and stored for future reference. Many enhancement methods have been reported for DAB involving different buffer conditions, the addition of metal ions and post-treatment applications. DAB has been used in immunohistochemical staining of nucleic acids and proteins. Application of the silver-gold intensified 3,3? -diaminobenzidine chromogen to the light and electron microscopic detection of the luteinizing hormone-releasing hormone system of the rat brain. 3, 3?-diaminobenzidine (DAB)-H2O2-horseradish peroxidase (HRP) has been presented and used for the sensitive detection of carcinoembryonic antigen (CEA) in human serum.
Zs. Liposits,; Gy. Sétaló, B. Flerkó.Application of the silver-gold intensified 3,3? -diaminobenzidine chromogen to the light and electron microscopic detection of the luteinizing hormone-releasing hormone system of the rat brain . Neuroscience. 1984, 13 (2), 513-525.
Volker Herzog,; H.Dariush Fahimi. A new sensitive colorimetric assay for peroxidase using 3,3?-diaminobenzidine as hydrogen donor . Analytical Biochemistry. 1973 , 55 (2), 554-562.
Mentions de danger (UE): H341-H350
Suspected of causing genetic defects. May cause cancer.
Mentions de prudence: P201-P280-P202-P308+P313-P405-P501a
Obtain special instructions before use. Wear protective gloves/protective clothing/eye protection/face protection. Do not handle until all safety precautions have been read and understood. IF exposed or concerned: Get medical advice/attention. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.