Alkaline Phosphatase, calf intestine, EIA GradeDephosphorylation of cloning vector DNA to prevent recircularization during ligation, dephosphorylation of DNA prior to end-labeling using T4 Polynucleotide Kinase, treatment of dNTPs in PCR reactions prior to sequencing or SNP analysis, dephosphorylation of DNA and RNA. It is also used to remove the 5?-terminal phosphate from nucleic acids during molecular cloning reactions.
C Wittmann., B Hock. Development of an ELISA for the analysis of atrazine metabolites deethylatrazine and deisopropylatrazine. J. Agric. Food Chem. 1991, 39,(6), 1194-1200.