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39450-01-6 - Proteinase K, Tritirachium album limber - J62051 - Alfa Aesar

J62051 Proteinase K, Tritirachium album limber

Numéro CAS
39450-01-6
Synonymes

Dimensions Prix ($) Quantité Disponibilité
25mg 47,80
100mg 101,00
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Proteinase K, Tritirachium album limber

MDL
MFCD00132129
EINECS
254-457-8

Propriétés chimiques

Forme
Lyophilized powder, 28.9 kDa
Solubilité
It is fully miscible with water.

Applications

Proteinase K is commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. Addition of Proteinase K to nucleic acid preparations rapidly inactivates nucleases that might otherwise degrade the DNA or RNA during purification. Proteinase K is specifically formulated for molecular cloning, nucleic acid research and protein/peptide structural analysis, inactivation of Rnase and Dnases, isolation of high molecular weight DNA, isolation of RNA or the isolation of plasmid and genomic DNA. Proteinase K is used for the isolation of native high molecular genomic nucleic acids.

Notes

Minimum 20 units per mg dry weight. One unit releases one micromole of Folin positive amino acids, measured as tyrosine, at 37 C and pH 7.5, using urea denatured hemoglobin as the substrate

Références bibliographiques

Betzel C.; Singh TP.; Visanji M.; Peters K.; Fittkau S.; Saenger W.; Wilson KS. Structure of the complex of proteinase K with a substrate analogue hexapeptide inhibitor at 2.2-A resolution.   J. Biol. Chem. 1993, 268, (21), 15854-8.

Müller A.; Hinrichs W.; Wolf WM.; Saenger W. Crystal structure of calcium-free proteinase K at 1.5-A resolution. J. Biol. Chem. 1994, 269, (37), 23108-11.

Mentions de danger et de prudence du SGH

Mentions de danger (UE): H334-H315-H319-H335

May cause allergy or asthma symptoms or breathing difficulties if inhaled. Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.

Mentions de prudence: P285-P261-P280-P305+P351+P338-P304+P340-P342+P311-P362-P312-P405-P501a

In case of inadequate ventilation wear respiratory protection. Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves/protective clothing/eye protection/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician. Take off contaminated clothing and wash before reuse. Call a POISON CENTER or doctor/physician if you feel unwell. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.

Autres références

Code tarifaire harmonisé
3507.90
TSCA
No

Recommandé

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Catalyseurs

Analytique et matériel de laboratoire