Ribonuclease B is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease B is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA. Ribonuclease B from bovine pancreas has been used in a study to assess hybridase activity of human ribonuclease-1. Ribonuclease B from bovine pancreas has also been used in a study to investigate particle-based and monolithic columns for cation exchange protein displacement chromatography.
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W Norde.; J Lyklema. The adsorption of human plasma albumin and bovine pancreas ribonuclease at negatively charged polystyrene surfaces: I. Adsorption isotherms. Effects of charge, ionic strength, and temperature. Journal of Colloid and Interface Science. 1978, 66, (2), 257-265.