Tween-20 is a non-ionic surfactant that is useful for solubilizing membrane proteins during isolation. Tween-20 is also added to buffers for immunohistochemistry. Tween-20 may also help to prevent non-specific antibody binding and function as a blocking agent in immunoblotting assays. It has been used in preextraction of membranes to remove peripheral proteins.
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Eric Lecuyer.; Neela Parthasarathy.; Henry M. Krause. Fluorescent In Situ Hybridization Protocols in Drosophila Embryos and Tissues. Drosophila.2008, 420, 289-302.