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9002-07-7 - Trypsin 1:250, porcine pancreas - EC 3.4.21.4 - J63993 - Alfa Aesar

J63993 Trypsin 1:250, porcine pancreas

Numéro CAS
9002-07-7
Synonymes
EC 3.4.21.4

Dimensions Prix ($) Quantité Disponibilité
10g 83,69
50g 199,63
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Trypsin 1:250, porcine pancreas

MDL
MFCD01775455
EINECS
232-650-8

Propriétés chimiques

Forme
Powder
Solubilité
Soluble in Hank's Balanced Salt Solution (35 mg/ml), HCl (1 mM), and water. Insoluble in clycerol and alcohol.

Applications

Trypsin 1:250 is used in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. It has also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestions. Additional applications include assessing crystallization by membrane-based techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps.

Notes

Low endotoxin. Minimum 225 units/mg. One unit causes a decrease in absorbance at 253 nm of 0.003 per minute at 25C.

Références bibliographiques

Paul P.Geurink; Wouter A van der Linden; Anne C.Mirabella; Nerea Gallastegui; Gerjan de Bruin; Annet E.M.Blom; Mathias J.Voges; Elliot D.Mock; Bogdan I.Florea; Gijs A van der Marel; Christoph Driessen; Mario van der Stelt; Michael Groll; Herman S.Overkleeft; Alexei F.Kisselev. Incorporation of non-natural amino acids improves cell permeability and potency of specific inhibitors of proteasome trypsin-like sites. Journal of medicinal and pharmaceutical chemistry. 2013, 56,(3), 1262-1275.

 

Simone A.Beckham; Jason Brouwer; Anna Roth; Die Wang; Anthony J.Sadler; Matthias John; Kerstin Jahn-Hofmann; Bryan R.G.Williams; Jacqueline A.Wilce; Matthew C.J.Wilce. Conformational rearrangements of RIG-I receptor on formation of a multiprotein:dsRNA assembly. Nucleic Acids Research. 2013, 41,(5), 3436-3445.

Mentions de danger et de prudence du SGH

Mentions de danger (UE): H334-H315-H319-H335

May cause allergy or asthma symptoms or breathing difficulties if inhaled. Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.

Mentions de prudence: P285-P261-P280-P305+P351+P338-P304+P340-P342+P311-P362-P312-P405-P501a

In case of inadequate ventilation wear respiratory protection. Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves/protective clothing/eye protection/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician. Take off contaminated clothing and wash before reuse. Call a POISON CENTER or doctor/physician if you feel unwell. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.

Autres références

Merck
14,9795
Code tarifaire harmonisé
3507.90
TSCA
Yes
RTECS
YN5075000

Recommandé

Consulté récemment

Produits chimiques

Sciences de la vie

Métaux et matériaux

Catalyseurs

Analytique et matériel de laboratoire