Albumin is used in protease-sensitive Immunoassays such as RIA, EIA, Fluorescent and Chemiluminescent, Protein Standard, Diluent, Protein, Conjugate or Enzyme Stabilizer. Bovine Serum Albumin is applicable for numerous biochemical applications including ELISAs (Enzyme-Linked Immunosorbent Assay), immunoblots, and immunohistochemistry. Frequently used as a blocking reagent; particularly useful with casein-sensitive antibodies, such as phospho-specific antibodies. Also used as a nutrient in cell and microbial culture. In restriction digests, BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. Commonly used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA. It is also used in hybridization and selected Cell Culture applications.
Naomi Kraus-Friedmann. A comparison between 45Ca2+ and atomic absorption in calcium flux determinations in perfused rat liver. Cell Calcium. 1987, 8, (3), 241-245.
Cole, W.G. Respiratory sequels to non-thoracic injury . The lancet. 1972, 299, (7750), 555-557.