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9004-07-3 - alpha-Chymotrypsin, from bovine pancreas - EC 3.4.21.1 - J65266 - Alfa Aesar

J65266 alpha-Chymotrypsin, from bovine pancreas

Numéro CAS
9004-07-3
Synonymes
EC 3.4.21.1

Dimensions Prix ($) Quantité Disponibilité
100microg 169,00
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alpha-Chymotrypsin, from bovine pancreas

MDL
MFCD00130481
EINECS
232-671-2

Propriétés chimiques

Poids formulaire
25kDa
Forme
Lyophilized powder
Solubilité
Reconstitute in 1mM HCl. Soluble at 10mg/ml in 1mM HCl. 2mM calcium chloride serves as a stabilizer. Store aliquoted solutions at -20°C for up to a week.

Applications

α-Chymotrypsin from bovine pancreas is used for protein identification by mass spectrometry, the isolation and characterization of myosin heavy chains, the incubation of infected and uninfected cells for analysis of cellular proteins by SDS-PAGE. A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.

Notes

Suitable for protein sequencing. 3X crystallized and treated with TLCK to inhibit trypsin. Dialyzed to remove autolysis products and low molecular weight contaminants. One unit with hydrolyze one micromole of benzoyl-L-tyrosine ethyl ester per minute at 25°C, pH 7.8.

Références bibliographiques

Tessa E.F. Quax; Mart Krupovic; Soizick Lucas; Patrick Forterre; David Prangishvili; Tessa E.F. Quax; Mart Krupovic; Soizick Lucas; Patrick Forterre; David Prangishvili. Structure of the Bovine Antimicrobial Peptide Indolicidin Bound to Dodecylphosphocholine and Sodium Dodecyl Sulfate Micelles. Virology. 2010, 404, (1), 1-4.

Miguel Ribeiro; Emmanuelle Bancel; Annie Faye; Mireille Dardevet; Catherine Ravel; Gerard Branlard; Gilberto Igrejas. Proteogenomic Characterization of Novel x-Type High Molecular Weight Glutenin Subunit 1Ax1.1. International Journal of Molecular Sciences. 2013, 14, (3), 5650-5667.

Mentions de danger et de prudence du SGH

Mentions de danger (UE): H334-H315-H319-H335

May cause allergy or asthma symptoms or breathing difficulties if inhaled. Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.

Mentions de prudence: P285-P261-P280-P305+P351+P338-P304+P340-P342+P311-P362-P312-P405-P501a

In case of inadequate ventilation wear respiratory protection. Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves/protective clothing/eye protection/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician. Take off contaminated clothing and wash before reuse. Call a POISON CENTER or doctor/physician if you feel unwell. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.

Autres références

Code tarifaire harmonisé
3507.90
TSCA
Yes
RTECS
GC3050000

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Consulté récemment

Produits chimiques

Sciences de la vie

Métaux et matériaux

Catalyseurs

Analytique et matériel de laboratoire