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31282-04-9 - Hygromycin B, 50 mg/ml in distilled water, sterile-filtered - J67371 - Alfa Aesar

J67371 Hygromycin B, 50 mg/ml in distilled water, sterile-filtered

Numéro de CAS
31282-04-9
Synonymes

Conditionnement Prix ($) Quantité Disponibilité
1ml 30,90
5x1ml 124,00
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Hygromycin B, 50 mg/ml in distilled water, sterile-filtered

MDL
MFCD06795479
EINECS
250-545-5

Propriétés chimiques

Forme
Liquid
Solubilité
Fully miscible in water and also soluble in methanol or ethanol and buffer solution.

Applications

Hygromycin B was used for hygro resistance effect in the following cells transfected 293T cells with mixture of plasmids at 160 ?g/ml, MCF-7ALOX12 cells at 150 ?g/ml, MSCV Luciferase PGK-hygro plasmid and STAT3 Reporter HeLa Stable Cell Line (stably transfected with Luciferase gene under the control of STAT3 promoter) maintained in DMEM supplemented with 10% FBS. It is suitable for the selection of cells transfected with Escherichia coli hygromycin resistance gene (hyg or hph), as well as for the maintenance of the Hygromycin phenotype of resistant cells. Consistent with its more specific effect on translocation, hygromycin B exhibits monophasic inhibition of polypeptide synthesis, suggesting that it binds to a single site on the ribosome.

Notes

Protect from heat. Keep the container tightly closed and place it in a cool, dry and well ventilated condition. Store in freezer at -20°C.

Références bibliographiques

Rao RN, et. al. Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli.Antimicrob Agents Chemother.,1983,24(5), 689-95.

González A, E, et al. Studies on the mode of action of hygromycin B, an inhibitor of translocation in eukaryotes.Biochim Biophys Acta.,1978,521(2), 459-69.

Mentions de danger et de prudence du SGH

Mentions de danger (UE): H301-H310-H330-H318-H334-H317

Toxic if swallowed. Fatal in contact with skin. Fatal if inhaled. Causes serious eye damage. May cause allergy or asthma symptoms or breathing difficulties if inhaled. May cause an allergic skin reaction.

Mentions de prudence: P301+P310a-P304+P340-P305+P351+P338-P320-P330-P405-P501a

IF SWALLOWED: IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Specific treatment is urgent (see label). Rinse mouth. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.

Autres références

Classe de danger
6.1
Groupe d'emballage
II
Code tarifaire harmonisé
2941.90
TSCA
No
RTECS
WK2130000

Recommandé

  • J61278

    Puromycin dihydrochloride, 99%
  • J67140

    Zeocin™, 100 mg/ml in HEPES, sterile-filtered

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Analytique et matériel de laboratoire