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Protein precipitation reagent Trichloroacetic acid is used as a precipitating agent in biochemistry for precipitation of proteins, DNA and RNA. It is an active ingredient used for cosmetic treatments like chemical peels, tattoo removal and the treatment of warts including genital warts. It is also used to determine protein concentration and a decalcifier and fixative in microscopy.
Trihaloacetic acids react with aldehydes in DMSO at room temperature to give trihalomethyl carbinols: J. Chem. Soc., Perkin 2, 1247 (1984):
The acid and its Na salt (1:1) in DMF are also effective: Tetrahedron Lett., 33, 3435 (1992). Use of HMPA or DMI extends the scope to less reactive aldehydes: Synthesis, 327 (1990).
Malachová, A.; Štočková, L.; Wakker, A.; Varga, E.; Krska, R.; Michlmayr, H.; Adam, G.; Berthiller, F. Critical evaluation of indirect methods for the determination of deoxynivalenol and its conjugated forms in cereals. Anal. Bioanal. Chem. 2015, 407 (20), 6009-6020.
Young, M. S.; van Tran, K.; Goh, E.; Shia, J. C. A Rapid SPE-Based Analytical Method for UPLC/MS/MS Determination of Aminoglycoside Antibiotic Residues in Bovine Milk, Muscle, and Kidney. J. AOAC Int. 2014, 97 (6), 1737-1741.
Dichiarazioni di rischio (UE): H314-H351
Causes severe skin burns and eye damage. Suspected of causing cancer.
Dichiarazioni precauzionali: P260u-P303+P361+P353-P305+P351+P338-P301+P330+P331-P405-P501a
IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. Rinse skin with water/shower. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF SWALLOWED: Rinse mouth. Do NOT induce vomiting. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.