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477-73-6 - Safranine O - C.I. 50240 - 3,7-Diamino-2,8-dimethyl-5-phenylphenazinium chloride - B21674 - Alfa Aesar

B21674 Safranine O

Codice CAS
477-73-6
Sinonimi
C.I. 50240
3,7-Diamino-2,8-dimethyl-5-phenylphenazinium chloride

Dimensioni Prezzo ($) Quantità Disponibilità
10g 22,20
50g 54,40
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Safranine O

MDL
MFCD00011759
EINECS
207-518-8

Proprietà chimiche

Formula
C20H19ClN4
Peso formula
350.85
Solubilità
Soluble in water at 20°C, 50g/L. Soluble in alcohol.

Applicazioni

It is a redox and adsorption indicator. Employed as a biological stain used in histology, cytology, bacteriology. It is commonly used as a counter stain for gram-negative bacteria in the Gram stain procedure. It has been observed to bind to DNA at a pH 2.21. In the Flemming triple stain procedure it is used to stain for chromosomes. In late Paleozoic pteridosperm cuticles it has shown increased contrast, emphasized cutinization differences, and enhanced the three-dimensional morphology of complex epidermal features when compared with other dyes. The compound has also been used with alcian blue in the histochemical study of the distribution and morphometric characteristics of mast cells. It has also been used to detect mucin and cartilage.

Note

Store away from oxidizing agents. Keep the container tightly closed and place it in a cool, dry and well ventilated condition.

Letteratura

Krings M. The use of biological stains in the analysis of late Paleozoic pteridosperm cuticles.Rev Palaeobot Palynol.2000108(3-4), 143-150.

Rosenberg L. Chemical basis for the histological use of safranin O in the study of articular cartilage.J Bone Joint Surg Am.197153(1), 69-82.

Biological stain.

Rischio GHS e dichiarazioni precauzionali

Dichiarazioni di rischio (UE): H315-H319-H335

Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.

Dichiarazioni precauzionali: P261-P280-P305+P351+P338-P304+P340-P362-P312-P321-P405-P403+P233-P501a

Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves/protective clothing/eye protection/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. Take off contaminated clothing and wash before reuse. Call a POISON CENTER or doctor/physician if you feel unwell. Specific treatment (see label). Store locked up. Store in a well-ventilated place. Keep container tightly closed. Dispose of contents/container in accordance with local/regional/national/international regulations.

Altri riferimenti

Beilstein
3924099
Codice tariffe armonizzato
2933.99
TSCA
Yes
RTECS
SG1623000

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