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Tris is used in pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications.Tris is used in assays used to characterize the activity and kinetics of the enzymes that catalyze SUMOylation of Small ubiquitin-like proteins (SUMO) and SUMO-dependent protein-protein interactions. Also used as buffers in a wide variety of biological systems. It has been used as a starting material for polymers, oxazolones (with carboxylic acids) and oxazolidines.
S. David Klein; Roger G. Bates. Conductance of tris(hydroxymethyl)-aminomethane hydrochloride (Tris·HCl) in water at 25 and 37°C. Journal of Solution Chemistry, 1980, 9(4), 289-292.
Titus H.J. Huisman; Andrée M. Dozy. Studies on the heterogeneity of hemoglobin : IX. The use of tris(hydroxymethyl)aminomethaneHCl buffers in the anion-exchange chromatography of hemoglobins. Journal of Chromatography A, 1965,19,160-169.