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5625-37-6 - PIPES, 1.0M buffer soln., pH 8.0 - J60618 - Alfa Aesar

J60618 PIPES, 1.0M buffer soln., pH 8.0

Codice CAS
5625-37-6
Sinonimi

Dimensioni Prezzo ($) Quantità Disponibilità
250ml 96,41
500ml 124,80
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PIPES, 1.0M buffer soln., pH 8.0

MDL
MFCD00006159

Proprietà chimiche

Modulo
Liquid
Solubilità
Fully misicible in water. Soluble in water (0.05 M), giving a clear, colorless solution (the resulting solution has a pH of approximately 4.5-4.7). 10 g will dissolve in 40 mL of 0.1 N NaOH.

Applicazioni

PIPES has pKa near the physiological pH which makes it useful in cell culture work. PIPES was used in the preparation of a variety of marine and mammalian tissues for electron microscopic study. Lipid loss and artifacts are minimized when PIPES was used to buffer the glutaraldehyde fixative. Fixation of fungal zoospores for fluorescence microscopy and electron microscopy was optimal with a combination of glutaraldehyde and formaldehyde in PIPES buffer. Alkaline phosphatase retention activity was 60% greater.

Note

Store in cool place. Keep container tightly closed in a dry and well-ventilated place. Store away from strong oxidizing agents, bases, reducing agents, heat.

Letteratura

P. S. Baur,; T. R. Stacey.The use of PIPES buffer in the fixation of mammalian and marine tissues for electron microscopy. Journal of Microscopy. 1977 , 109 (3), 315-327.

K. Yamamoto,; K. Ogawa. Effects of NaOH-PIPES buffer used in aldehyde fixative on alkaline phosphatase activity in rat hepatocytes. Histochemistry. 1983, 77 (3), 339-351.

Altri riferimenti

Codice tariffe armonizzato
2933.59
TSCA
Yes

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