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9001-75-6 - Pepsin, porcine stomach - EC 3.4.23.1 - Pepsin A - J61679 - Alfa Aesar

J61679 Pepsin, porcine stomach

Codice CAS
9001-75-6
Sinonimi
EC 3.4.23.1
Pepsin A

Dimensioni Prezzo ($) Quantità Disponibilità
1g 58,70
5g 174,00
10g 285,00
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Pepsin, porcine stomach

MDL
MFCD00081840
EINECS
232-629-3

Proprietà chimiche

Modulo
Powder
Solubilità
Soluble in water and dilute aqueous buffers

Applicazioni

Pepsin is commonly used in the preparation of Fab fragments from antibodies. Pepsin, from porcine gastric mucosa, has been used to hydrolyze dry cervical samples in mice. has been used in the digestion of crude wheat gliadin. It has been used along with other enzymes to demonstrate the effects of fixation and enzymatic digestion in immunohistochemical assays, using paraffin embedded tissue. It has been used for digestion (before using immunoperoxidase techniques) to reduce non-specific background staining in sections of bronchial tissues. The enzyme has also been used in the preparation of F(ab)2 fragment from IgG.

Note

Minimum 2,500 units/mg. One unit increases absorbance at 280nm by 0.001 per minute at 37 C and pH 2.0, measured as TCA soluble products from denatured hemoglobin

Letteratura

TJ McDonald.; G Nilsson.; M Vagne.; M Ghatei. A gastrin releasing peptide from the porcine nonantral gastric tissue. Gut . < span>1978, 19, (2), 767-774.

J Jancarik.; SH Kim. Sparse matrix sampling: a screening method for crystallization of proteins. J. Appl. Cryst. < span>1978, 24, 409-411.

Rischio GHS e dichiarazioni precauzionali

Dichiarazioni di rischio (UE): H315-H319-H334-H335

Causes skin irritation. Causes serious eye irritation. May cause allergy or asthma symptoms or breathing difficulties if inhaled. May cause respiratory irritation.

Dichiarazioni precauzionali: P284-P261-P280a-P305+P351+P338-P304+P340-P342+P311a-P312a-P405-P403+P233-P501a

Wear respiratory protection. Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves and eye/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. If experiencing respiratory symptoms: Store locked up. Store in a well-ventilated place. Keep container tightly closed. Dispose of contents/container in accordance with local/regional/national/international regulations.

Altri riferimenti

Merck
14,7146
Codice tariffe armonizzato
3507.90
TSCA
Yes
RTECS
SC6132000

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