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Elastase is used in combination with other proteases for protein analysis by mass spectrometry. Elastase, is used in tissue dissociation because elastin is found in highest concentrations in the elastic fibers of connective tissues, elastase is frequently used to dissociate tissues that contain extensive intercellular fiber networks. It is usually used with other enzymes such as collagenase, trypsin, and chymotrypsin, membrane protein solubilization, protein sequence studies.
RD Hautamaki.; DK Kobayashi.; RM Senior.; SD Shapiro. Requirement for macrophage elastase for cigarette smoke-induced emphysema in mice. Science. 1997, 277, (5334), 2002-2004 .
J Bieth.; B Spiess.; CG Wermuth. The synthesis and analytical use of a highly sensitive and convenient substrate of elastase. Biochemical medicine. 1974, 11, (4), 350-357.
Dichiarazioni di rischio (UE): H334
May cause allergy or asthma symptoms or breathing difficulties if inhaled.
Dichiarazioni precauzionali: P284-P261-P342+P311a-P304+P341-P501a
Wear respiratory protection. Avoid breathing dust/fume/gas/mist/vapours/spray. If experiencing respiratory symptoms: Call a POISON CENTER/doctor. IF INHALED: If breathing is difficult, remove to fresh air and keep at rest in a position comfortable for breathing. Dispose of contents/container in accordance with local/regional/national/international regulations.