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This enzyme can be used alone or in combination with other proteases for protein analysis by mass spectrometry and other applications. Elastase, is used in tissue dissociation because elastin is found in highest concentrations in the elastic fibers of connective tissues, elastase is frequently used to dissociate tissues that contain extensive intercellular fiber networks. For this purpose, it is usually used with other enzymes such as collagenase, trypsin, and chymotrypsin, membrane protein solubilization, protein sequence studies.
RD Hautamaki.; DK Kobayashi.; RM Senior.; SD Shapiro. Requirement for macrophage elastase for cigarette smoke-induced emphysema in mice. Science. 1997, 277, (5334), 2002-2004 .
J Bieth.; B Spiess.; CG Wermuth. The synthesis and analytical use of a highly sensitive and convenient substrate of elastase. Biochemical medicine. 1974, 11, (4), 350-357.
Dichiarazioni di rischio (UE): H315-H319-H334-H335
Causes skin irritation. Causes serious eye irritation. May cause allergy or asthma symptoms or breathing difficulties if inhaled. May cause respiratory irritation.
Dichiarazioni precauzionali: P284-P261-P280a-P305+P351+P338-P405-P501a
Wear respiratory protection. Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves and eye/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.