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Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA.
Ribonuclease A from bovine pancreas has been used in a study to assess hybridase activity of human ribonuclease-1. Ribonuclease A from bovine pancreas has also been used in a study to investigate particle-based and monolithic columns for cation exchange protein displacement chromatography.
Chromatographically purified. Minimum 3000 units/mg dry wt. One unit causes an increase in absorbance of 1.0 at 260nm at 37 C and pH 5.0 when yeast ribosomal RNA is hydrolyzed to acid soluble oliognucleotides.
This product does not need to be activated. The heat treatment of RNase is usually performed to remove traces of DNase, however, heating solutions of RNase A to inactivate DNase may not be satisfactory since RNase activity may be lost if precipitate formation occurs and heat treated DNase may reactivate over time.
Can be used without treatment in some applications.
To heat-treat, use 10mM acetate pH 5.0 with or without 15mM CaCl2 for 15 minutes at 100°C or longer at 80°C. Product may precipitate if heated at neutral pH.
Soluble in water or acetate buffer and Tris buffer may also work. Aliquots can be frozen at 1 mg/ml in water.
Nana Asare et. al. 1-Nitropyrene (1-NP) induces apoptosis and apparently a non-apoptotic programmed cell death (paraptosis) in Hepa1c1c7 cells. Toxicology and Applied Pharmacology. 2008, 230, (2), 175-186.
W Norde.; J Lyklema. The adsorption of human plasma albumin and bovine pancreas ribonuclease at negatively charged polystyrene surfaces: I. Adsorption isotherms. Effects of charge, ionic strength, and temperature. Journal of Colloid and Interface Science. 1978, 66, (2), 257-265.