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39450-01-6 - Proteinase K, Tritirachium album limber - J62051 - Alfa Aesar

J62051 Proteinase K, Tritirachium album limber

Codice CAS
39450-01-6
Sinonimi

Dimensioni Prezzo ($) Quantità Disponibilità
25mg 47,80
100mg 101,00
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Proteinase K, Tritirachium album limber

MDL
MFCD00132129
EINECS
254-457-8

Proprietà chimiche

Modulo
Lyophilized powder, 28.9 kDa
Solubilità
It is fully miscible with water.

Applicazioni

Proteinase K is commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. Addition of Proteinase K to nucleic acid preparations rapidly inactivates nucleases that might otherwise degrade the DNA or RNA during purification. Proteinase K is specifically formulated for molecular cloning, nucleic acid research and protein/peptide structural analysis, inactivation of Rnase and Dnases, isolation of high molecular weight DNA, isolation of RNA or the isolation of plasmid and genomic DNA. Proteinase K is used for the isolation of native high molecular genomic nucleic acids.

Note

Minimum 20 units per mg dry weight. One unit releases one micromole of Folin positive amino acids, measured as tyrosine, at 37 C and pH 7.5, using urea denatured hemoglobin as the substrate

Letteratura

Betzel C.; Singh TP.; Visanji M.; Peters K.; Fittkau S.; Saenger W.; Wilson KS. Structure of the complex of proteinase K with a substrate analogue hexapeptide inhibitor at 2.2-A resolution.   J. Biol. Chem. 1993, 268, (21), 15854-8.

Müller A.; Hinrichs W.; Wolf WM.; Saenger W. Crystal structure of calcium-free proteinase K at 1.5-A resolution. J. Biol. Chem. 1994, 269, (37), 23108-11.

Rischio GHS e dichiarazioni precauzionali

Dichiarazioni di rischio (UE): H315-H319-H334-H335

Causes skin irritation. Causes serious eye irritation. May cause allergy or asthma symptoms or breathing difficulties if inhaled. May cause respiratory irritation.

Dichiarazioni precauzionali: P284-P261-P280a-P305+P351+P338-P405-P501a

Wear respiratory protection. Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves and eye/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.

Altri riferimenti

Codice tariffe armonizzato
3507.90
TSCA
No

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