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145224-94-8 - MES, 0.5M buffer soln., pH 5.0 - J62081 - Alfa Aesar

J62081 MES, 0.5M buffer soln., pH 5.0

Codice CAS
145224-94-8
Sinonimi

Dimensioni Prezzo ($) Quantità Disponibilità
100ml 48,20
250ml 56,16
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MES, 0.5M buffer soln., pH 5.0

MDL
MFCD00283848

Proprietà chimiche

Modulo
Liquid
Solubilità
Soluble in water (0.5 M at 20°C).

Applicazioni

MES, is often used as a running buffer for resolving very small proteins on bis-tris gels, also used as a biological buffer in plant cell cultures. It is also been used in various protein characterization assays. This sterile solution suitable for cell culture and molecular biology applications. It is also used as a buffering agent in biology and biochemistry.

Note

Solutions are stable at 2-8°C for months. Sterilize by filtration through 0.2 mm filters. Autoclaving is not recommended for any sulfonic acid buffer. If buffers must be nuclease-free, treat the water first, then add the buffer after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown.

Letteratura

BG Bugbee.; FB Salisbury. An evaluation of MES (2 (N-Morpholino) ethanesulfonic acid) and amberlite IRC-50 as pH buffers for nutrient solution studies. Journal of plant nutrition. 1985, 8, ( 7), 567-583.

MJ Alowitz.; MM Scherer . Kinetics of nitrate, nitrite, and Cr (VI) reduction by iron metal. Environmental Science & Technology. 2002, 36, (3), 299-306.

Rischio GHS e dichiarazioni precauzionali

Dichiarazioni di rischio (UE): H315-H319

Causes skin irritation. Causes serious eye irritation.

Dichiarazioni precauzionali: P280-P264-P305+P351+P338-P362-P321-P332+P313-P337+P313-P302+P352

Wear protective gloves/protective clothing/eye protection/face protection. Wash thoroughly after handling. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Take off contaminated clothing and wash before reuse. Specific treatment (see label). If skin irritation occurs: Get medical advice/attention. If eye irritation persists: IF ON SKIN: Wash with plenty of soap and water.

Altri riferimenti

Codice tariffe armonizzato
2934.99
TSCA
Yes

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