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9003-98-9 - Deoxyribonuclease I, bovine pancreas - EC 3.1.21.1 - DNase I - J62229 - Alfa Aesar

J62229 Deoxyribonuclease I, bovine pancreas

Codice CAS
9003-98-9
Sinonimi
EC 3.1.21.1
DNase I

Dimensioni Prezzo ($) Quantità Disponibilità
25mg 51,00
100mg 132,00
1g 1002,00
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Deoxyribonuclease I, bovine pancreas

MDL
MFCD00130918
EINECS
232-667-0

Proprietà chimiche

Modulo
Lyophilized powder, 31 kDa
Solubilità
It is soluble in 0.15 M NaCl.

Applicazioni

Deoxyribonuclease I is used for the removal of DNA from protein samples. DNAse I is used to nick DNA as a first step to incorporate labeled bases into DNA. The enzyme from Sigma has been used during the isolation of plasma membrane vesicles from Neurospora crassa cell culture.1 It has also been used along with trypsin for the preparation of single cell suspension from rat testes. Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis

Note

Minimum 2,000 units per mg dry weight. One unit causes an increase in absorbance at 260nm of 0.001 per minute per ml at 25 degrees when acting upon highly polymerized DNA at pH 5.0

Letteratura

G A Scarborough. The neurospora plasma membrane ATPase is an electrogenic pump. PNAS. 1976, 73 (5), 1485-1488.

U Lindberg. Molecular weight and amino acid composition of deoxyribonuclease I. Biochemistry. 1967, 6 (1), 335-342.

Rischio GHS e dichiarazioni precauzionali

Dichiarazioni precauzionali: P260-P201-P280-P304+P340-P405-P501a

Do not breathe dust/fume/gas/mist/vapours/spray. Obtain special instructions before use. Wear protective gloves/protective clothing/eye protection/face protection. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.

Altri riferimenti

Merck
14,2906
Codice tariffe armonizzato
3507.90
TSCA
Yes
RTECS
RF0750000

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