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9001-99-4 - Ribonuclease A, bovine pancreas, Molecular Biology Grade - EC 3.1.27.5 - Rnase A - J62232 - Alfa Aesar

J62232 Ribonuclease A, bovine pancreas, Molecular Biology Grade

Codice CAS
9001-99-4
Sinonimi
EC 3.1.27.5
Rnase A

Dimensioni Prezzo ($) Quantità Disponibilità
200mg 110,00
1g 440,00
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Ribonuclease A, bovine pancreas, Molecular Biology Grade

MDL
MFCD00132181
EINECS
232-646-6

Proprietà chimiche

Modulo
Lyophilized powder
Sensibilità
Moisture Sensitive
Solubilità
Reconstitute in 10mM acetate, pH 5.0 with or without 15mM calcium chloride

Applicazioni

Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA. Ribonuclease A from bovine pancreas has been used in a study to assess hybridase activity of human ribonuclease-1. Ribonuclease A from bovine pancreas has also been used in a study to investigate particle-based and monolithic columns for cation exchange protein displacement chromatography.

Note

Minimum 2500 units/mg dry wt. Chromatographically purified. Heat treat at 100°C for 15 minutes to inactivate DNAase. One unit causes an increase in absorbance of 1.0 at 260nm at 37 C and pH 5.0 when yeast ribosomal RNA is hydrolyzed to acid soluble oligonucleotides.

Letteratura

Nana Asare et. al. 1-Nitropyrene (1-NP) induces apoptosis and apparently a non-apoptotic programmed cell death (paraptosis) in Hepa1c1c7 cells. Toxicology and Applied Pharmacology. 2008, 230, (2), 175-186.

W Norde.; J Lyklema. The adsorption of human plasma albumin and bovine pancreas ribonuclease at negatively charged polystyrene surfaces: I. Adsorption isotherms. Effects of charge, ionic strength, and temperature. Journal of Colloid and Interface Science. 1978, 66, (2), 257-265.

Rischio GHS e dichiarazioni precauzionali

Dichiarazioni precauzionali: P260u-P201-P280-P303+P361+P353-P305+P351+P338-P301+P330+P331-P304+P340-P310a-P405-P501a

Obtain special instructions before use. Wear protective gloves/protective clothing/eye protection/face protection. IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. Rinse skin with water/shower. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF SWALLOWED: Rinse mouth. Do NOT induce vomiting. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.

Altri riferimenti

Merck
14,8202
Codice tariffe armonizzato
3507.90
TSCA
Yes
RTECS
RF0760000

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