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TBE (10X), TRIS + borate + EDTA - J62449 - Alfa Aesar

J62449 TBE (10X), TRIS + borate + EDTA

Codice CAS
Sinonimi

Dimensioni Prezzo ($) Quantità Disponibilità
1L 27,30
2L 37,44
4L 88,37
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TBE (10X), TRIS + borate + EDTA

MDL
MFCD00236358

Proprietà chimiche

Modulo
Liquid
Solubilità
It is soluble in water.

Applicazioni

TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. TBE Buffer is used for polyacrylamide and agarose gel electrophoresis. It is ideally suited for DNA and RNA separation on longer run, higher voltage or amperage gels. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel.5 TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. Tris-Borate-EDTA buffer has been used for pulsed-field gel electrophoresis (PFGE).

Note

0.9M Tris base, 0.9M boric acid, 20mM EDTA

Letteratura

Brody, J.R.; Kern, S.E. History and principles of conductive media for standard DNA electrophoresis. Anal Biochem . 2004, 333, (1), 1-13.

L Orbán and A Chrambach. Discontinuous buffer system for polyacrylamide and agarose gel electrophoresis of DNA fragments. Electrophoresis. 1991, 12, (4), 233-240.

Rischio GHS e dichiarazioni precauzionali

Dichiarazioni di rischio (UE): H360-H315-H319

May damage fertility or the unborn child. Causes skin irritation. Causes serious eye irritation.

Dichiarazioni precauzionali: P201-P280-P281-P305+P351+P338-P362-P308+P313-P321-P332+P313-P405-P501a

Obtain special instructions before use. Wear protective gloves/protective clothing/eye protection/face protection. Use personal protective equipment as required. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Take off contaminated clothing and wash before reuse. IF exposed or concerned: Get medical advice/attention. Specific treatment (see label). If skin irritation occurs: Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.

Altri riferimenti

Codice tariffe armonizzato
3822.00
TSCA
Yes

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