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Neutral Red is most commonly used for histological stain for lysosymes but has also been incorporated in investigation of viruses, as a pH indicator, determination of DNA with optical and electrochemical methods, evaluation of synthetic and naturally derived biomaterials. Neutral red can be electrochemically polymerized and used as a redox mediator. The compound once taken up by cells can be used to acertain cell viability with a excitation maximum at 470 nm and emission maximum at 580nm. It is used as a red nuclear counter stain in various cytochemical procedures. It is suitable for microscopy and microbiology and supravital staining of living blood cells. A vital dye used as an indicator and biological stain.
Antal, P., et al. Flow cytometric assay of phagocytic activity of human neutrophils and monocytes in whole blood by neutral red uptake. Ann. Hematol. 1995, 70, (5), 259-65.
Ricci, V., et al. Extracellular pH modulates Helicobacter pylori-induced vacuolation and VacA toxin internalization in human gastric epithelial cells. Biochem. Biophys. Res. Commun. 2002, 292, (1), 167-74.