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TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. TBE Buffer is used for polyacrylamide and agarose gel electrophoresis. It is ideally suited for DNA and RNA separation on longer run, higher voltage or amperage gels. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel.5 TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. Tris-Borate-EDTA buffer has been used for pulsed-field gel electrophoresis (PFGE).
Brody, J.R.; Kern, S.E. History and principles of conductive media for standard DNA electrophoresis. Anal Biochem . 2004, 333, (1), 1-13.
L Orbán and A Chrambach. Discontinuous buffer system for polyacrylamide and agarose gel electrophoresis of DNA fragments. Electrophoresis. 1991, 12, (4), 233-240.
Dichiarazioni di rischio (UE): H315-H319-H360
Causes skin irritation. Causes serious eye irritation. May damage fertility or the unborn child.
Dichiarazioni precauzionali: P201-P280-P305+P351+P338-P308+P313-P405-P501a
Obtain special instructions before use. Wear protective gloves/protective clothing/eye protection/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF exposed or concerned: Get medical advice/attention. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.