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DNA ladders are used in gel electrophoresis to determine the size and quantity of testing DNA fragments of genomic, plasmid, and PCR DNA. It is commonly used in the studies of plasmid DNA and PCR products. 1 kb DNA Ladder has been used as a molecular marker to determine the molecular weight and size of double stranded DNA during electrophoresis. 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb, and 2 kb repeats from 6 to 10 kb. In NA electrophoresis, five ?l of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. Suitable for use in Northern and Southern blotting. All DNA markers can be stained with ethidium bromide, SYBR® Green, and Nancy-520.
T Asahida.; T Kobayashi.; K Saitoh.; I Nakayama. Tissue preservation and total DNA extraction from fish stored at ambient temperature using buffers containing high concentration of urea. Fisheries Science. 199662 (5), 727-730.
J Welsh.; M McClelland. Fingerprinting genomes using PCR with arbitrary primers. Nucleic acids research. 199018 (24), 7213-7218.
Dichiarazioni di rischio (UE): H319
Causes serious eye irritation.
Dichiarazioni precauzionali: P280i-P264-P305+P351+P338-P337+P313
Wear eye/face protection. Wash thoroughly after handling. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice/attention.