Informativa e consenso per l'uso dei cookie

Accetto Il nostro sito salva piccoli pezzi di informazioni (cookie) sul dispositivo, al fine di fornire contenuti migliori e per scopi statistici. È possibile disattivare l'utilizzo di cookies modificando le impostazioni del tuo browser. Continuando la navigazione si acconsente all'utilizzo dei cookie.

39450-01-6 - Proteinase K, Ready-to-Use soln. - J63710 - Alfa Aesar

J63710 Proteinase K, Ready-to-Use soln.

Codice CAS
39450-01-6
Sinonimi

Dimensioni Prezzo ($) Quantità Disponibilità
5ml 232,00
25ml 917,00
Aggiungi al carrello Aggiungi al preventivo generale Visualizza articolo

Proteinase K, Ready-to-Use soln.

MDL
MFCD00132129
EINECS
254-457-8

Proprietà chimiche

Modulo
Liquid, 28.9 kDa
Solubilità
Miscible with water.

Applicazioni

A stable and highly reactive serine protease; used for protein and nucleic acid isolation.Proteinase K is used in the purification of RNA and DNA from tissues or cell lines. It finds application in protein modification, determination of enzyme localization and inactivation of RNases/DNases during nucleic acid extraction. It is also used in mitochondria isolation and removal of endotoxins bound to cationic proteins like lysozyme and ribonuclease A. Further, it is involved in the treatment of paraffin embedded tissue sections in order to expose antigen binding sites for antibody labeling.

Note

Minimum 400 units per ml. Protein concentration is 20 mg/ml in 10mM Tris-HCl, 1mM calcium acetate, pH 7.5 containing 50% glycerol. RNase and DNase free. One unit releases one micromole of Folin positive amino acids, measured as tyrosine, at 37°C and pH 7.5, using urea denatured hemoglobin as the substrate.Incompatible with strong oxidizing agents. Store in a cool place.

Letteratura

Fränzel, B.; Fischer, F.; Steegborn, C.; Wolters, D. A. Proteinase K improves quantitative acylation studies. Proteomics 2015, 15 (1), 44-47.

Shishova, K. V.; Khodarovich, Y. M.; Lavrentyeva, E. A.; Zatsepina, O. V. Analysis of the localization of fibrillarin and sites of pre-rRNA synthesis in the nucleolus-like bodies of mouse GV oocytes after mild treatment with proteinase K. Russ. J. Dev. Biol. 2015, 46 (3), 127-136.

Rischio GHS e dichiarazioni precauzionali

Dichiarazioni di rischio (UE): H334-H319

May cause allergy or asthma symptoms or breathing difficulties if inhaled. Causes serious eye irritation.

Dichiarazioni precauzionali: P285-P261-P280-P264-P305+P351+P338-P342+P311-P304+P341-P337+P313-P501a

In case of inadequate ventilation wear respiratory protection. Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves/protective clothing/eye protection/face protection. Wash thoroughly after handling. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician. IF INHALED: If breathing is difficult, remove to fresh air and keep at rest in a position comfortable for breathing. If eye irritation persists: Get medical advice/attention. Dispose of contents/container in accordance with local/regional/national/international regulations.

Altri riferimenti

Codice tariffe armonizzato
3507.90
TSCA
No

Raccomandato

  • J60872

    Miconazole, 99%
  • A14887

    3-Hydroxy-2-nitropyridine, 98%
  • H31551

    2-Methoxy-4-(trifluoromethyl)phenylacetic acid, 97%
  • H53214

    Potassium 2-methylphenyltrifluoroborate, 96%
  • 43191

    Copper Manganese Nickel wire, 1.0mm (0.04in) dia

Visualizzati di recente

Prodotti chimici

Scienze della vita

Metalli e materiali

Catalizzatori

Apparecchiature di analisi e di laboratorio