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A stable and highly reactive serine protease; used for protein and nucleic acid isolation.Proteinase K is used in the purification of RNA and DNA from tissues or cell lines. It finds application in protein modification, determination of enzyme localization and inactivation of RNases/DNases during nucleic acid extraction. It is also used in mitochondria isolation and removal of endotoxins bound to cationic proteins like lysozyme and ribonuclease A. Further, it is involved in the treatment of paraffin embedded tissue sections in order to expose antigen binding sites for antibody labeling.
Fränzel, B.; Fischer, F.; Steegborn, C.; Wolters, D. A. Proteinase K improves quantitative acylation studies. Proteomics 2015, 15 (1), 44-47.
Shishova, K. V.; Khodarovich, Y. M.; Lavrentyeva, E. A.; Zatsepina, O. V. Analysis of the localization of fibrillarin and sites of pre-rRNA synthesis in the nucleolus-like bodies of mouse GV oocytes after mild treatment with proteinase K. Russ. J. Dev. Biol. 2015, 46 (3), 127-136.
Dichiarazioni di rischio (UE): H319-H334
Causes serious eye irritation. May cause allergy or asthma symptoms or breathing difficulties if inhaled.
Dichiarazioni precauzionali: P284-P261-P280i-P305+P351+P338-P342+P311a-P501a
Wear respiratory protection. Avoid breathing dust/fume/gas/mist/vapours/spray. Wear eye/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If experiencing respiratory symptoms: Dispose of contents/container in accordance with local/regional/national/international regulations.