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39450-01-6 - Proteinase K, Ready-to-Use soln. - J63710 - Alfa Aesar

J63710 Proteinase K, Ready-to-Use soln.

Codice CAS
39450-01-6
Sinonimi

Dimensioni Prezzo ($) Quantità Disponibilità
5ml 232,00
25ml 917,00
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Proteinase K, Ready-to-Use soln.

MDL
MFCD00132129
EINECS
254-457-8

Proprietà chimiche

Modulo
Liquid, 28.9 kDa
Solubilità
Miscible with water.

Applicazioni

A stable and highly reactive serine protease; used for protein and nucleic acid isolation.Proteinase K is used in the purification of RNA and DNA from tissues or cell lines. It finds application in protein modification, determination of enzyme localization and inactivation of RNases/DNases during nucleic acid extraction. It is also used in mitochondria isolation and removal of endotoxins bound to cationic proteins like lysozyme and ribonuclease A. Further, it is involved in the treatment of paraffin embedded tissue sections in order to expose antigen binding sites for antibody labeling.

Note

Minimum 400 units per ml. Protein concentration is 20 mg/ml in 10mM Tris-HCl, 1mM calcium acetate, pH 7.5 containing 50% glycerol. RNase and DNase free. One unit releases one micromole of Folin positive amino acids, measured as tyrosine, at 37°C and pH 7.5, using urea denatured hemoglobin as the substrate.Incompatible with strong oxidizing agents. Store in a cool place.

Letteratura

Fränzel, B.; Fischer, F.; Steegborn, C.; Wolters, D. A. Proteinase K improves quantitative acylation studies. Proteomics 2015, 15 (1), 44-47.

Shishova, K. V.; Khodarovich, Y. M.; Lavrentyeva, E. A.; Zatsepina, O. V. Analysis of the localization of fibrillarin and sites of pre-rRNA synthesis in the nucleolus-like bodies of mouse GV oocytes after mild treatment with proteinase K. Russ. J. Dev. Biol. 2015, 46 (3), 127-136.

Rischio GHS e dichiarazioni precauzionali

Dichiarazioni di rischio (UE): H319-H334

Causes serious eye irritation. May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Dichiarazioni precauzionali: P284-P261-P280i-P305+P351+P338-P342+P311a-P501a

Wear respiratory protection. Avoid breathing dust/fume/gas/mist/vapours/spray. Wear eye/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If experiencing respiratory symptoms: Call a POISON CENTER/doctor. Dispose of contents/container in accordance with local/regional/national/international regulations.

Altri riferimenti

Codice tariffe armonizzato
3507.90
TSCA
No

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