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Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality.
Wiesława Zaborska.; Barbara Krajewska.; Maciej Leszko.; Zofia Olech. Inhibition of urease by Ni2+ ions: Analysis of reaction progress curves. Journal of Molecular Catalysis B: Enzymatic. 2001, 13, (4-6), 103-108.
Adam Juszkiewicz.; Anna Zaborska.; Anna Łaptaś.; Zofia Olech. A study of the inhibition of jack bean urease by garlic extract. Food Chemistry. 2004, 85, (4), 553-558.