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J65104 Guanidine thiocyanate, Molecular Biology Grade

Codice CAS
593-84-0
Sinonimi
Guanidine rhodanide

Stock No. Dimensioni Prezzo ($) Quantità Disponibilità
J65104-18 50g 75,30
J65104-30 250g 157,00
J65104-36 500g 266,00
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Guanidine thiocyanate, Molecular Biology Grade

MDL
MFCD00013027
EINECS
209-812-1

Proprietà chimiche

Formula
CH5N3.CHNS
Peso formula
118.16
Modulo
Crystalline solid
Punto di fusione
118-122°
Densità
1.29
Storage & Sensitivity
Light Sensitive. Ambient temperatures.
Solubilità
Soluble in water (1420 g/L) at 20°C, and ethanol (5%).

Applicazioni

Guanidine thiocyanate is used for RNA isolation and can solubilize proteins. When the compound is used to solubilize proteins it can be removed by dialysis against urea without difficulty. The hybridization of guanidine thiocyanate-solubilize hepatocytes can be used as a method to quantify mRNA from hepatocytes. Blood sample lysate stored in guanidine thiocyanate has been shown to maintain nucleic acid integrity. Research describes guanidine thiocyanate to be ineffective for RNA isolation of plant tissue with a high levels of secondary metabolites and storage proteins.

Note

Light Sensitive. Keep container tightly closed. Store away from oxidizing agents.

Letteratura

Gregory J. Wadsworth.; Margaret G. Redinbaugh.; John G. Scandalios. A procedure for the small-scale isolation of plant RNA suitable for RNA blot analysis . Analytical Biochemistry. 1988, 172, (1), 279-283.

John M. Chirgwin.; Alan E. Przybyla.; Raymond J. MacDonald.; William J. Rutter. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979, 18, (24), 5294-5299.

Rischio GHS e dichiarazioni precauzionali

Dichiarazioni di rischio (UE): H302-H312-H332

Harmful if swallowed. Harmful in contact with skin. Harmful if inhaled.

Dichiarazioni precauzionali: P261-P280h-P304+P340-P301+P312a-P321-P501a

Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves/clothing. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell. Specific treatment (see label). Dispose of contents/container in accordance with local/regional/national/international regulations.

Altri riferimenti

Beilstein
3563461
Codice tariffe armonizzato
2925.29
TSCA
Yes
RTECS
XL1225000

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