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75621-03-3 - CHAPS, Electrophoresis Grade - 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate - J67359 - Alfa Aesar

J67359 CHAPS, Electrophoresis Grade

Codice CAS
75621-03-3
Sinonimi
3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate

Dimensioni Prezzo ($) Quantità Disponibilità
5g 86,57
10g 175,88
25g 429,00
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CHAPS, Electrophoresis Grade

MDL
MFCD00012116

Proprietà chimiche

Formula
C32H58N2O7S
Peso formula
614.88
Modulo
Powder
Punto di fusione
156-158°
Sensibilità
Hygroscopic
Solubilità
Soluble in water (10 mg/ml). Soluble in DMF and hot methanol.

Applicazioni

CHAPS is often used as a detergent in the solubilization and purification of membrane proteins for several advantageous reasons. CHAPS detergent is non-denaturing to membrane proteins, can solubilize proteins, disaggregate protein-protein interactions. CHAPS is also useful in ion exchange chromatography and isoelectric focusing as it is zwitterionic and does not exhibit a net charge between pH 2 to 12. It is suitable for isolation of membrane proteins with the following features such as sufficient protein solubilization capability, no denaturing or inactivation of proteins, no interference with protein activities, no precipitation at 4°C, appropriate critical micelle concentrations (CMC) and micelle size, no absorption in the UV region, no toxicity and availability of detergent detection methods.

Note

Hygroscopic. Store away from water/moisture and oxidizing agents. Keep the container tightly closed and place it in a cool, dry and well ventilated condition. Store under inert gas.

Letteratura

Jacob Anglister, et. al. Isotope-edited multidimensional NMR of calcineurin B in the presence of the non-deuterated detergent CHAPS.J. Biomol. NMR.,1993,3(1), 121-126.

F. Anne Stephenson and Richard W. Olsen. Solubilization by CHAPS Detergent of Barbiturate-Enhanced Benzodiazepine-GABA Receptor Complex.J. Neurochem.,1982,39(6), 1579-1586.

Rischio GHS e dichiarazioni precauzionali

Dichiarazioni di rischio (UE): H315-H319-H335

Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.

Dichiarazioni precauzionali: P261-P280-P305+P351+P338-P304+P340-P362-P312-P321-P405-P403+P233-P501a

Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves/protective clothing/eye protection/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. Take off contaminated clothing and wash before reuse. Call a POISON CENTER or doctor/physician if you feel unwell. Specific treatment (see label). Store locked up. Store in a well-ventilated place. Keep container tightly closed. Dispose of contents/container in accordance with local/regional/national/international regulations.

Altri riferimenti

Codice tariffe armonizzato
2924.29
TSCA
No

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