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2-Succinimido-1,1,3,3-tetramethyluronium tetrafluoroborate (TSTU) acts as an efficient coupling agent through rapid activation of carboxylic acids to form N-succinimidyl active esters, which react with primary amines to form carboxamides. It is effective even in the presence of water. TSTU is selective towards amines in the presence of alcoholic group. It is employed as a coupling regent in the synthesis of peptides. With amine-protected alpha-amino acids, it brings about coupling without significant epimerization. It is useful for linking of sensor molecules with amino groups to amino-functionalized AFM (atomic force microscope) tips.
Yang, Q.; Wang, H.; Lan, X.; Cheng, B.; Chen, S.; Shi, H.; Xiao, H.; Ma, Y. Reflection-mode micro-spherical fiber-optic probes for in vitro real-time and single-cell level pH sensing. Sens. Actuators B 2015, 207, 571-580.
Yang, Q.; Wang, H.; Chen, S.; Lan, X.; Xiao, H.; Shi, H.; Ma, Y. Fiber-Optic-Based Micro-Probe Using Hexagonal 1-in-6 Fiber Configuration for Intracellular Single-Cell pH Measurement. Anal. Chem. 2015, 87 (14), 7171-7179.
Dichiarazioni di rischio (UE): H301-H315-H319-H335
Toxic if swallowed. Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.
Dichiarazioni precauzionali: P261-P280a-P301+P310a-P305+P351+P338-P405-P501a
Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves and eye/face protection. IF SWALLOWED: Immediately call a POISON CENTER/doctor IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.