ADA used to prepare immobilized pH gradients. It can be used to study biological buffers and zwitterionic compounds. It has been used in a study to describe the application of scanning capacitively coupled contactless conductivity detection (SC(4)D) for the determination of pH dependant behaviour of two aminopolycarboxylates immobilized onto the surface of a monolithic capillary column.
P.G.Righetti; M.Chiari; C.Gelfi. Immobilized pH gradients: effect of salts, added carrier ampholytes and voltage gradients on protein patterns. Electrophoresis. 1988, 9,(2), 65-73.
Ting-Chien Chen; Andrew Hong. Chelating extraction of lead and copper from an authentic contaminated soil using N-(2-acetamido)iminodiacetic acid and S-carboxymethyl-l-cysteine. Journal of Hazardous Materials. 1995, 41,(2-3), 147-160.
Biological buffer, pKa = 6.9 at 20o: Biochemistry, 5, 467 (1966).