HEPES [N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)] is a general purpose buffer for biological research. Its applications include, tissue culture, Phosphorylation and photophosphorylation, fixative Transmission electron microscopy, protein synthesis, preventing binding to non-receptor materials.
H Sands .; LJ Gorey-Feret .; AJ Cocuzza .; FW Hobbs. Biodistribution and metabolism of internally 3H-labeled oligonucleotides. I. Comparison of a phosphodiester and a phosphorothioate. Molecular Pharmacology. 1994, 45, (5), 932-943.
DH Lee .; SY Kim .; JI Hong. A fluorescent pyrophosphate sensor with high selectivity over ATP in water. Angewandte Chemie International Edition. 1994, 43, (36), 4777-4780.