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B21034 5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside, 98+%

CAS 번호
7240-90-6
동의어
X-gal

Stock No. 사이즈 가격 ($) 수량 재고 여부
B21034-MA 10mg 29.90
B21034-MF 50mg 59.60
B21034-MD 250mg 192.00
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5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside, 98+%

MDL
MFCD00005666
EINECS
230-640-8

화학적 특성

화학식
C14H15BrClNO6
화학 식량
408.64
융점
ca 236° dec.
Storage & Sensitivity
Store under Nitrogen. Keep Cold. Moisture Sensitive. Light Sensitive.
용해도
Soluble in 100 mM in DMSO.

용도

It is a substrate for beta-galactosidase, which cleaves the glycosidic bond to give 5-bromo-4-chloro-3-hydroxy-1H-indole, which immediately dimerises to give an intensely blue product. It is also used to detect activity of this enzyme in histochemistry and bacteriology. It is employed as a colorless, endogenous or exogenous pigment precursors that may be transformed by biological mechanisms into colored compounds. They are used in biochemical assays and in diagnosis as indicators, particularly in the form of enzyme substrates. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.

참고

Moisture & Light sensitive. Store away from oxidizing agents, heat, water/moisture and light. Store under inert gas. Keep the container tightly closed and place it in a cool, dry and well ventilated condition.

참조 문헌

Christiaen L, et al. X-gal staining of electroporated sea squirt (Ciona) embryos.Cold Spring Harbor protocols.20092009(12), prot5346.

Kim MY, et al. X-gal inhibits the swarming of Vibrio species.J. Microbiol. Methods.200666(3), 552-555.

기타 참조

바일슈타인
1402009
통합 관세 코드
2934.99
TSCA(독성물질규제법)"
No

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